HPLC Column, RP PFP, 3um, 2.1mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68322‑10P‑2 Cogent RP PFP™ HPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, delivering improved resolution where traditional C18 phases may not fully separate analytes.
This stationary phase utilizes a pentafluorophenyl (PFP, “F5”) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, providing multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These combined effects allow for greater selectivity control and improved separation of structurally similar compounds.
The 100 Å pore size provides versatility across a wide range of analytes, making it suitable for both small and moderately sized molecules in LC‑MS workflows.
100 mm Length – Increased Resolution for LC‑MS Methods
The 100 mm length provides greater resolving power than 50 mm columns, improving separation while maintaining efficient run times.
Better resolution of closely related compounds
Increased peak capacity for complex samples
Improved selectivity for difficult separations
✅ Use when additional resolution is required beyond fast screening methods
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
⚠️ Requires low‑flow HPLC or LC‑MS system capability
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Higher backpressure vs 5 µm columns
Compatible with low‑volume LC‑MS detectors
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity compared to C18
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in method development
Versatile 100 Å Pore Structure
Suitable for a wide range of analytes
Balanced performance across applications
Cogent RP PFP™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry for polar analytes
High Efficiency (3 µm Particles)
More efficient than 5 µm columns
Sharper peaks and improved resolution
High Purity Silica
Consistent chromatographic performance
Reliable reproducibility across runs
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical analytical workflows
LC‑MS method development
USP L43 method applications
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for low‑flow LC‑MS and analytical HPLC systems (2.1 mm ID)
Higher backpressure than larger particle size columns
Use 0.2 µm filtration to maintain column performance